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MOUZAKI ATHANASIA, Professor

MOUZAKI ATHANASIA

Sector: Division of Internal Medicine Ι

Clinic/Laboratory: Department of Internal Medicine

Knowledge Subject: Laboratory Hematology and Transfusion Medicine

Research Area: Immunology (molecular, cellular, clinical), Immunohematology, Hematology

Laboratory of Immunohematology, Division of Hematology, Dpt. of Internal Medicine, Medical School, University of Patras, Rion, Patras GR-26500, Greece Build. A, 1st floor, west wing

+30-2610-969123 mouzaki@upatras.gr athanasia.mouzaki@gmail.com mouzaki@med.upatras.gr

Download Curriculum Vitae

Summary of Curriculum Vitae

1977: High School Diploma from 2nd Lyceon for Girls (Patras, Greece). 1979: General Certificates of Education(GCEs) in Chemistry, Physics, Mathematics and English (Cambridge Board, UK). 1983: Bachelor's degree (B.Sc./Honors) in Biological Sciences (Major: Physiology, Specialization: Cellular Toxicology) (University of Plymouth, UK). Diploma thesis: “Response of the lymphoid organs of the laboratory mouse to Plasmodium yoelli infection”. 1985: Master's degree (MSc.) in Biological Sciences (Immunology) (University of Salford, UK). MSc thesis: “Gasterosteus aculeatus, Linnaeus: The lymphoid system, diseases and ecological correlates”. 1988: Doctor of Philosophy (Ph.D.) (University of Salford, UK and Free University of Berlin, DE). Ph.D. thesis: “The rat receptor for Interleukin-2”. Positions/Teaching appointments: 1983-1985: Part-time lecturer and demonstrator, Laboratory of Immunology, University of Salford, Greater Manchester, UK. 1985-1987: Research assistant, Institute of Immunology, Medical School, Klinikum Steglitz, Berlin, Germany. 1987-1990: Assistante post-doctorante, Dpt. of Molecular Biology, University of Geneva, Geneva, Switzerland. 1991-1992: Assistante post-doctorante, Dpt. of Hematology, Medical School and University Hospital, Geneva, Switzerland. 1992-1995: Maitre Assistante, Dpt. of Hematology, Medical School and University Hospital, Geneva, Switzerland. 1995-1997: Lecturer of Laboratory Hematology and Transfusion Medicine, Medical School, University of Patras, Patras, Greece. 1997-2003: Assistant Professor of Laboratory Hematology and Transfusion Medicine, Medical School, University of Patras, Patras, Greece. 2003-2009: Associate Professor of Laboratory Hematology and Transfusion Medicine, Division of Hematology, Dpt. of Internal Medicine, Medical School, University of Patras, Greece. Current appointment: Professor of Laboratory Hematology and Transfusion Medicine, Division of Hematology, Dpt. of Internal Medicine, Medical School, University of Patras, Greece. Director of the Immunohematology Laboratory.

Undergraduate Courses

Postgraduate Courses

1996-now: Participation in post-graduate courses of the post-graduate program “Basic Medical Sciences” (Faculty of Medicine, University of Patras). Teaching in:

Research methodology [28h]-coordinator of unit II

How to write scientific papers [4h]

Current topics of biological research [4h]-coordinator (1/2)

Immunochemistry [8h]

Cellular immunology [8h]

Practical training in Immunohematology and Immunology [40h]

1997-now: Participation in the post-graduate program “Medicinal Chemistry” (Faculty of Medicine and Dpt. of Chemistry, University of Patras). Teaching the unit: “Basic and Translational Immunology” [26h]

2001-2011: Participation in the post-graduate program of the Dpt. of Biology, Univ. of Patras. Teaching the unit: “The immune response” [20h]

2004-2011: Participation in the post-graduate program “Clinical and Laboratory Medicine” (Faculty of Medicine, University of Patras). Teaching the unit: “How to write science papers” [4h]

Selected Publications

Di Stefano R, Mouzaki A, Araneda D, Diamanstein T, Tilney NL, Kupiec-Weglinski JW. Anti-interleukin 2 receptor monoclonal antibodies spare phenotypically distinct T suppressor cells in vivo and exert synergistic biological effects. J. Exp. Med. 1988, 167:1981-1986.

Mouzaki A, Weil R, Muster L, Rungger D. Silencing and trans-activation of the mouse IL-2 gene in Xenopus oocytes by proteins from resting and mitogen-induced primary T-lymphocytes. EMBO J. 1991, 10(6):1399-1406.

Hentsch B, Mouzaki A, Pfeuffer I, Rungger D, Serfling E. The weak, fine-tuned binding of ubiquitous transcription factors to the IL-2 enhancer contributes to its T cell-restricted activity. Nucleic Acids Res. 1992, 20(11):2657-2665.

Mouzaki A, Rungger D. Properties of transcription factors regulating interleukin 2 gene transcription through the NFAT binding site in untreated or drug-treated naïve and memory T helper cells. Blood 1994, 84(8):2612-2621.

Mouzaki A, Serfling E, Zubler RH. Interleukin-2 promoter activity in Epstein-Barr virus-transformed B lymphocytes is controlled by nuclear factor-κB. Eur. J. Immunol. 1995, 25:2177-2182.

Mouzaki A, Matthes T, Miescher PA, Beris P. Polyclonal hypergammaglobulinaemia in a case of B cell Chronic Lymphocytic Leukaemia: The result of IL-2 production by the proliferating monoclonal B cells? Br. J. Haematol. 1995, 91:345-349.

Mouzaki A, Doucet A, Mavroidis E, Muster L, Rungger D. A repression-derepression mechanism regulating the transcription of human immunodeficiency virus type 1 in primary T cells. Mol. Med. 2000, 6(5):377-390.

Mouzaki A, Theodoropoulou M, Gianakopoulos I, Vlaha V, Kyrtsonis M-C, Maniatis A. Expression Patterns of Th1 and Th2 Cytokine Genes in Childhood Idiopathic Thrombocytopenic Purpura (ITP) at Presentation and their Modulation by Intravenous Immunoglobulin G (IVIg) Treatment; their Role in Prognosis. Blood 2002, 100(5):1774-1779.

Panitsas F, Theodoropoulou M, Kouraklis A, Karakantza M, Theodorou G, Zoumbos NC, Maniatis A, Mouzaki A. Adult chronic idiopathic thrombocytopenic purpura (ITP) is the manifestation of a type-1 polarized immune response. Blood 2004, 103(7):2645-2647.

Argyropoulos C, Nikiforidis GC, Theodoropoulou M, Adamopoulos P, Boubali S, Georgakopoulos TN, Paliogianni F, Papavassiliou AG, Mouzaki A. Mining microarray data to identify transcription factors expressed in naïve resting but not activated T lymphocytes. Genes Immun. 2004, 5(1):16-25.

Chatzantoni K, Papathanassopoulos P, Gourzoulidou E, Mouzaki A. Leptin and its soluble receptor in plasma of patients suffering from remitting-relapsing multiple sclerosis (MS). In vitro effects of leptin on type-1 and type-2 cytokine secretion by peripheral blood mononuclear cells, T-cells and monocytes of MS patients. J. Autoimmun. 2004, 23(2):169-177.

Deraos G, Chatzantoni K, Matsoukas M-T, Tselios T, Deraos S, Katsara M, Papathanasopoulos P, Vynios D, *Apostolopoulos V, *Mouzaki A, *Matsoukas J. Citrullination of linear and cyclic altered peptide ligands from Myelin Basic Protein (MBP87-99) epitope elicits a Th1 polarized response by T cells isolated from multiple sclerosis patients: implications in triggering disease. J. Med. Chem. 2008, 51(24):7834-7842. [*senior authors]

Mouzaki A, Panagoulias I, Dervilli Z, Zolota V, Spadidea P, Rodi M, Panitsas FP, Lagadinou E, de Lastic A-L, Georgakopoulos T. Expression patterns of leptin receptor (OB-R) isoforms and direct in vitro effects of recombinant leptin on OB-R, leptin expression and cytokine secretion by human hematopoietic malignant cells. Cytokine 2009, 48:203-211.

Varvarigou AA, Thomas I, Rodi M, Economou I, Mantagos S, Mouzaki A. Respiratory distress syndrome (RDS) in premature infants is underscored by the magnitude of Th1 cytokine polarization. Cytokine 2012, 58(3):355-360.

Mouzaki A, Panagoulias I, Raptis G, Farri-Kostopoulou E. Cord blood leptin levels of healthy neonates are associated with IFN-γ production by cord blood T-cells. PLoS One 2012, 7(7):e4083086.

Mouzaki A, Rodi M, Dimisianos N, Emmanuil A, Kalavrizioti D, Lagoudaki R, Grigoriadis NC, Papathanasopoulos P. Immune parameters that distinguish multiple sclerosis patients from patients with other neurological disorders at presentation. PLoS One 2015, 10(8):e0135434.

de Lastic AL, Rodi M, Mouzaki A. Effect of dendritic cell state and antigen-presentation conditions on resulting T-cell phenotypes and Th cytokine profiles. Immunobiology 2016, 221(8):862-870.

Panagoulias I, Georgakopoulos T, Aggeletopoulou I, Agelopoulos M, Thanos D, Mouzaki A. Transcription Factor Ets-2 Acts as a Pre-induction Repressor of Interleukin-2 (IL-2) Transcription in Naive T Helper Lymphocytes. J. Biol. Chem. 2016, 291(52):26707-26721.

Panagoulias I, Karagiannis F, Aggeletopoulou I, Georgakopoulos T, Argyropoulos CP, Akinosoglou K, Gogos C, Skoutelis A, Mouzaki A. Ets-2 acts as a transcriptional repressor of the human immunodeficiency virus type 1 through binding to a repressor-activator target sequence of 5’-LTR. Front. Immunol. 2018, 8(JAN):1924

Psianou K, Panagoulias I, Papanastasiou AD, de Lastic A-L, Rodi M, Spantidea PI, Degn SE, Georgiou P, Mouzaki A. Clinical and immunological parameters of Sjögren’s syndrome. Autoimmun. Rev. 2018, 17(10):1053-1064.

Selected Presentations

Μay 1988. 19th International Leukocyte Culture Conference, Banf, Alberta, Canada. Title of presentation: In vivo action of anti-IL2R mAbs.

Μarch 1992. Invited speaker in a meeting organized by the University of Geneva and Glaxo Institute for Molecular Biology (Geneva). Title of presentation: Regulation of the IL-2 gene in T-cell subsets.
Oct. 1994. Invited speaker in a symposium on “Transcription Factors in Immunology”. Title of presentation: IL-2 gene regulation through the NFAT promoter element in peripheral blood human T cells: A binding site of pleiotropic transcription factors that change radically with the acquisition of memory by T cells. Munich, Germany.
April 1995. Invited speaker in the 2nd EUCAMBIS (European Union Concerted Action on Molecular Biology of Immunosenescence) meeting, Tuebingen, Germany. Title of presentation: Aspects of IL-2 gene regulation in T- and B-cells of healthy and diseased individuals.
July 1998. ISH-EHA Combined Haematology Congress, Amsterdam, Holland. Title of presentation: Abnormal cytokine gene expression in thrombocytopenic purpura is partially restored after immunoglobulin administration.
July 2000. 13th International Symposium on Treatment of Leukemia, Lymphoma & Cancer. Molecular Biology of Hematopoiesis. New York City, NY, USA. Title of presentation: Expression of apoptotic genes in multiple myeloma cells.
Μάρτ.2007. Invited speaker in the 5th Seminar of the Hellenic Society of Transfusion Medicine, Athens, Greece. Title of presentation: Immunomodulation by blood transfusion.
Dec. 2011. 2nd International Meeting of the Development and Assessment of Multipotent Antimicrobial and Antiinflammatory Drugs, Patras, Greece. Title of presentation: Ets-2 is a transcriptional repressor of the IL-2 gene in naive T helper cells and a key molecule in autoimmune diseases
June 2012. Joint meeting of the Israeli and Greek Societies of Neuroimmunology, Chalkidiki, Greece. Invited speaker. Title of talk: Tregs in RR-MS: Few but functional.
Sept. 2013. 21st Meeting of the Hellenic College of Nephrology, Patras, Greece. Invited speaker. Title of talk: The role of FoxP3+ T regulatory cells in renal transplantation: Myths and reality.
Nov. 2014. 1st European Workshop on Tolerogenic Vaccination in Autoimmune Diseases, Novara, Italy. Invited speaker. Title of talk: Effect of dendritic cells state and antigen presentation conditions on resulting T cell phenotypes and Th cytokine profiles.
Dec. 2018. 2nd Annual meeting on Cancer biology and New Molecules in Cancer Therapeutics, Athens, Greece. Invited speaker. Title of talk: Autoimmunity and cancer.
 

PhD Theses Advisor

Supervisor in Ph.D. theses (completed):

The role of complement in liver regeneration. By D. Mastellos. Presented 21.12.2000. Medical School, Univ. of Patras.

Th1 and Th2 cytokines in autoimmune diseases. By K. Chatzantoni. Presented 25.6.2003. Medical School, Univ. of Patras.

Role of pro- and anti-apoptotic genes in the pathogenesis of multiple myeloma. By I. Xagoraris. Presented 30.6.2005. Medical School, Univ. of Patras.

Effect of immunosuppressive regimens on the type and function of T regulatory cells of patients that underwent kidney transplantation. By P. Dousdambanis. Presented 9.7.2010. Medical School, Univ. of Patras.

Determination of the levels of leptin and pro- and anti-inflammatory cytokines in neonates; investigation of putative involvement of elevated leptin levels in the pathogenesis of autoimmune diseases. By G. Raptis. Presented 27.7.2012. Medical School, Univ. of Patras.

Vaccine development for cancer using peptides that mimic HLA epitopes to present cancer antigens. By A. L. de Lastic. Presented 13.11.2012. Medical School, Univ. of Patras.

Cells and soluble mediators that influence the formation of heterotopic ossification following traumatic brain injury or musculoskeletal trauma. By P. Sakellaraki. Presented 28.5.2014. Medical School, Univ. of Patras.

Differential regulation of the expression of the interleukin-2 gene and the HIV-1 virus by transcriptional silencers in naive and memory helper T cells. By I. Panagoulias. Presented 25.7.2014. Medical School, Univ. of Patras.

Effect of allogeneic blood transfusion on factors that influence T helper cell plasticity. By P. Spantidea. Presented 21.12.2015. Medical School, Univ. of Patras.

Identification of transcription factors that regulate cytokine gene expression during T cell differentiation. By F. Karagiannis. Presented 12.5.2016. Medical School, Univ. of Patras.

Development of in vitro cell culture systems of dendritic cells for the presentation of artificial antigens to T cells from patients with multiple sclerosis for the future development of therapeutic vaccines. Presented by M. Rodi. Presented 19.6.2018. Medical School, Univ. of Patras.

Role of Tip60 in transcriptional regulation of cytokine genes in helper T cells. By I. Aggeletopoulou. Presented 19.7.2019. Medical School, Univ. of Patras.

Also:

Supervisor in another 4 Ph.D. Theses (ongoing), 40 MSc. theses (37 completed), 11 Diploma projects (completed). Participation in the examination committees of 53 Ph.D. theses and 16 MSc. theses.

Other Activities

Research grants: Recipient of 29 research grants (PI or co-PI). The total amount received by my laboratory from these grants = 1,253,996 €.

Member of 11 Scientific Societies. Founding member of the Hellenic Academy of Neuroimmunology.

Clinical duties: Responsible for the Laboratory of the Hellenic Center for Disease Control and Prevention (KEELPNO) in Patras, Greece. The laboratory measures the HIV viral load and performs HLA-B*5701 typing and genotypic analyses to determine drug-resistance and other mutations of individuals with established HIV-1 infection attending the Division of Infectious Diseases of the University Hospital, Patras, Greece. Responsible for the investigation of the prevalence of HIV-1 subtypes in southwestern Greece.

Administrative duties: Established the research laboratory of Immunohematology and participated in the setting and start-up of common research facilities of the Medical School, University of Patras. Member (1/5) of a committee appointed by the Health Ministry to design the application of molecular testing for viruses in the Blood Centers of Greece. Member (1/3) of a committee that runs the quality control of the post-graduate course “Biomedical Sciences” of the Medical School, University of Patras. Member of the General Assembly of the Medical School, University of Patras, for the acad. years 1995-96, 1998-99, 2001-02, 2006-07, 2011-12, 2015-19. Member (since 1995) of a committee that organizes and supervises the laboratory courses of the students of the Medical School, University of Patras. Head of the committee since 2009. Vice President of the post-graduate program Medicinal Chemistry & Biological Chemistry (Dpt. of Chemistry and Medical School, University of Patras) for acad. years 2018-19 and 2019-20. Member of electoral boards for the election of faculty in Hematology and Immunology in all Greek Universities and the Biomedical Research Foundation of the Academy of Athens, Athens, Greece.